<p>This data set consists on a raster line scan performed over HEK-293 cells expressing dimers of the fluorescent protein Venus, also known as SEYFP-F46L.
The scan line is 64 pixels long, and the scanning direction is from the cytoplasm to the nucleus, across the nuclear envelope.
A pixel size of 50 nm was used, as well as a pixel dwell time of 12.5 us and a line scan time of 1.925 ms.
Fluorescence excitation was provided by a 488 nm laser at 0.1
Fluorescence intensity data was collected using the photon-counting mode in an Olympus FV1000 Upright BX61WI confocal microscope.</p>

datasets


This is a package for fluorescence fluctuation spectroscopy data analysis methods such as spFCS, FCCS, scanning-FCS, pCF, N&B and pCOMB, among others.
In addition, several data detrending tools are provided. For an extensive user's guide for the use of FCSlib, please navigate to (<https://github.com/FCSlib/FCSlib/tree/master/Documentation>).
Sample data can be found at (<https://github.com/FCSlib/FCSlib/tree/master/Sample%20Data>).
The original paper where this package is presented can be found at (<doi:10.1093/bioinformatics/btaa876>).

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FCSlib

A Collection of Fluorescence Fluctuation Spectroscopy Analysis
Methods

V2 function

<p>A matrix with 64 rows and 25000 columns</p>

V2: Line-scan data of HEK-293 cells expressing Venus (EYFP) dimers

Description

Usage

Arguments

Format

Details