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NormqPCR (version 1.18.0)

Functions for normalisation of RT-qPCR data

Description

Functions for the selection of optimal reference genes and the normalisation of real-time quantitative PCR data.

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Version

Version

1.18.0

License

LGPL-3

Maintainer

James Perkins

Last Published

February 15th, 2017

Functions in NormqPCR (1.18.0)

Colon

Colon dataset of Andersen et al (2004)
BladderRepro

Dataset of Andersen et al (2004)
geNorm

Data set of Vandesompele et al (2002)
stabMeasureRho

Gene expression stability value rho
deltaDeltaCt

Perform normalization and differential expression with given housekeeping gene
Bladder

Bladder dataset of Andersen et al (2004)
combineTechReps

Combines Technical Replicates
deltaCt

Perform normalization with a given housekeeping gene
CqValues

Compute Cq value and amplification efficiency
ComputeNRQs

Compute Normalized Relative Quantities
combineTechRepsWithSD

Combines Technical Replicates
makeAllNewVal

Make all Cq values NA
geomMean

Geometric Mean
replaceNAs

Replace NAs with a given value
replaceAboveCutOff

Replace Cq values with new value
NormqPCR-package

Functions for normalisation of RT-qPCR data.
selectHKs

Selection of reference/housekeeping genes
makeAllNAs

Make all Cq values NA
stabMeasureM

Gene expression stability value M