NormqPCR (version 1.18.0)

replaceNAs: Replace NAs with a given value

Description

Replace NAs with a given value

Usage

replaceNAs(qPCRBatch, ...)
"replaceNAs"(qPCRBatch, newNA)

Arguments

qPCRBatch

Expression set containing qPCR data.

...

Extra arguments, detailed below

newNA

The new value to replace the NAs with

Value

qPCRBatch object with a new exprs slot

Details

Replaces NA values in the exprs slot of the qPCRBatch object with a given number

References

Perkins, JR, Dawes, JM, McMahon, SB, Bennett, DL, Orengo, C, Kohl, M (2012). ReadqPCR and NormqPCR: R packages for the reading, quality checking and normalisation of RT-qPCR quantification cycle (Cq) data. BMC Genomics, 13, 1:296.

Examples

  path <- system.file("exData", package = "NormqPCR")
  taqman.example <- file.path(path, "example.txt")
  qPCRBatch.taqman <- read.taqman(taqman.example)
  qPCRBatch.taqman.replaced <- replaceNAs(qPCRBatch.taqman, newNA = 40)
  exprs(qPCRBatch.taqman.replaced)["Ccl20.Rn00570287_m1",]