genArise (version 1.48.0)

global.norm: Global Normalization of Spot

Description

This function normalize R and I values and fit the value of Cy5 from his argument. In this function the normalize algorithm will be applied to all observations to get the lowess factor and then fit Cy5 with this factor. The observations. The observations with values R = 0 are deleted because they have no change in their expression levels.

Usage

global.norm(mySpot)

Arguments

mySpot

A spot object

Value

A new spot object but normalized, It means with a different Cy5 that is the result of the fit with the lowess factor.

Examples

data(Simon)
# Background Correction
c.spot <- bg.correct(Simon)
#Normalized data
n.spot <- global.norm(c.spot)