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pathfindR (version 2.4.2)

Enrichment Analysis Utilizing Active Subnetworks

Description

Enrichment analysis enables researchers to uncover mechanisms underlying a phenotype. However, conventional methods for enrichment analysis do not take into account protein-protein interaction information, resulting in incomplete conclusions. 'pathfindR' is a tool for enrichment analysis utilizing active subnetworks. The main function identifies active subnetworks in a protein-protein interaction network using a user-provided list of genes and associated p values. It then performs enrichment analyses on the identified subnetworks, identifying enriched terms (i.e. pathways or, more broadly, gene sets) that possibly underlie the phenotype of interest. 'pathfindR' also offers functionalities to cluster the enriched terms and identify representative terms in each cluster, to score the enriched terms per sample and to visualize analysis results. The enrichment, clustering and other methods implemented in 'pathfindR' are described in detail in Ulgen E, Ozisik O, Sezerman OU. 2019. 'pathfindR': An R Package for Comprehensive Identification of Enriched Pathways in Omics Data Through Active Subnetworks. Front. Genet. .

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install.packages('pathfindR')

Monthly Downloads

1,668

Version

2.4.2

License

MIT + file LICENSE

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Maintainer

Ege Ulgen

Last Published

February 17th, 2025

Functions in pathfindR (2.4.2)

enrichment_chart

Create Bubble Chart of Enrichment Results
filterActiveSnws

Parse Active Subnetwork Search Output File and Filter the Subnetworks
fuzzy_term_clustering

Heuristic Fuzzy Multiple-linkage Partitioning of Enriched Terms
enrichment_analyses

Perform Enrichment Analyses on the Input Subnetworks
get_kegg_gsets

Retrieve Organism-specific KEGG Pathway Gene Sets
enrichment

Perform Enrichment Analysis for a Single Gene Set
hierarchical_term_clustering

Hierarchical Clustering of Enriched Terms
get_mgsigdb_gsets

Retrieve Organism-specific MSigDB Gene Sets
gset_list_from_gmt

Retrieve Gene Sets from GMT-format File
get_pin_file

Retrieve Organism-specific PIN data
plot_scores

Plot the Heatmap of Score Matrix of Enriched Terms per Sample
get_reactome_gsets

Retrieve Reactome Pathway Gene Sets
pathfindR

pathfindR: A package for Enrichment Analysis Utilizing Active Subnetworks
single_iter_wrapper

Active Subnetwork Search + Enrichment Analysis Wrapper for a Single Iteration
run_pathfindR

Wrapper Function for pathfindR - Active-Subnetwork-Oriented Enrichment Workflow
get_biogrid_pin

Retrieve the Requested Release of Organism-specific BioGRID PIN
fetch_gene_set

Fetch Gene Set Objects
fetch_java_version

Obtain Java Version
score_terms

Calculate Agglomerated Scores of Enriched Terms for Each Subject
get_gene_sets_list

Retrieve Organism-specific Gene Sets List
summarize_enrichment_results

Summarize Enrichment Results
input_processing

Process Input
return_pin_path

Return The Path to Given Protein-Protein Interaction Network (PIN)
hyperg_test

Hypergeometric Distribution-based Hypothesis Testing
visualize_KEGG_diagram

Visualize Human KEGG Pathways
process_pin

Process Data frame of Protein-protein Interactions
visualize_active_subnetworks

Visualize Active Subnetworks
input_testing

Input Testing
isColor

Check if value is a valid color
term_gene_graph

Create Term-Gene Graph
visualize_term_interactions

Visualize Interactions of Genes Involved in the Given Enriched Terms
visualize_terms

Create Diagrams for Enriched Terms
term_gene_heatmap

Create Terms by Genes Heatmap
combined_results_graph

Combined Results Graph
check_java_version

Check Java Version
cluster_enriched_terms

Cluster Enriched Terms
combine_pathfindR_results

Combine 2 pathfindR Results
UpSet_plot

Create UpSet Plot of Enriched Terms
active_snw_search

Perform Active Subnetwork Search
annotate_term_genes

Annotate the Affected Genes in the Provided Enriched Terms
cluster_graph_vis

Graph Visualization of Clustered Enriched Terms
active_snw_enrichment_wrapper

Wrapper for Active Subnetwork Search + Enrichment over Single/Multiple Iteration(s)
color_kegg_pathway

Color hsa KEGG pathway
create_kappa_matrix

Create Kappa Statistics Matrix
configure_output_dir

Configure Output Directory Name
create_HTML_report

Create HTML Report of pathfindR Results