A dataset containing the \(C_T\) values of two genes from a serial dilution
qPCR experiment. The original dataset shows only the averages and standard
deviations of each of the 7 different dilutions (1, .5, .2, .1, .05, .02 and .01).
These summaries were used to regenerate 3 replicates for each of the dilutions
to be used in testing and examples of the different functions.

datasets

Calculates the amplification efficiency and curves from real-time
quantitative PCR (Polymerase Chain Reaction) data. Estimates the relative
expression from PCR data using the double delta CT and the standard curve
methods Livak & Schmittgen (2001) <doi:10.1006/meth.2001.1262>. Tests for
statistical significance using two-group tests and linear regression
Yuan et al. (2006) <doi: 10.1186/1471-2105-7-85>.

Mahmoud Ahmed

Analyzing Real-Time Quantitative PCR Data

ct3 function

A data.frame with 21 rows and 2 variables:<dl class="dl-horizontal">
 <dt>c_myc</dt><dd>\(C_T\) values of the target gene c-myc</dd>
 <dt>GAPDH</dt><dd>\(C_T\) values of the control gene GAPDH</dd>
</dl>

Format

\(C_T\) values from qPCR (Serial dilutions) — ct3

A data.frame with 21 rows and 2 variables:<dl class='dl-horizontal'>
 <dt>c_myc</dt><dd>\(C_T\) values of the target gene c-myc</dd>
 <dt>GAPDH</dt><dd>\(C_T\) values of the control gene GAPDH</dd>
</dl>

ct3: \(C_T\) values from qPCR (Serial dilutions)

Description

Usage

Arguments

Format

See Also