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strvalidator (version 1.3.0)

calculateOverlap_gui: Bins Overlap

Description

calculateOverlap_gui is a GUI wrapper for the calculateOverlap function.

Usage

calculateOverlap_gui(env = parent.frame(), savegui = NULL, debug = TRUE)

Arguments

env
environment in wich to search for data frames and save result.
savegui
logical indicating if GUI settings should be saved in the environment.
debug
logical indicating printing debug information.

Value

  • TRUE

Details

By analysis of the bins overlap between dye channels a measure of the risk for spectral pull-up artefacts can be obtain. The default result is a matrix with the total bins overlap in number of base pairs. If an allele frequency database is provided the overlap at each bin is multiplied with the frequency of the corresponding allele. If no frequence exist for that allele a frequency of 5/2N will be used. X and Y alleles is given the frequency 1. A scoring matrix can be supplied to reduce the effect by spectral distance, meaning that overlap with the neighbouring dye can be counted in full (100while a non neighbour dye get its overlap reduced (to e.g. 10

See Also

calculateOverlap