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synlet (version 1.2.2)

zFactor: Calcualte the Z and Z' factor

Description

calcualte the Z and Z' factor for each plate.

Usage

zFactor(dat, negativeCon, positiveCon, useMean = TRUE)

Arguments

dat
synthetic lethal RNAi screen data.
negativeCon
the negative control used in the WELL_CONTENT_NAME.
positiveCon
the positive control used in the WELL_CONTENT_NAME.
useMean
use mean to calcualate z factor and z' factor by default; otherwise use median.

Value

  • A data.frame contains z factor and z' factor

References

Zhang J.H., Chung T.D. & Oldenburg K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. B, 4 67-73 (1999). Birmingham,A. et al. (2009) Statistical methods for analysis of high-throughput RNA interference screens. Nat Methods, 6, 569-575.

Examples

Run this code
zFactor(exampleDat, negativeCon = "scrambled control si1", positiveCon = "PLK1 si1")

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