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synlet (version 1.2.2)

Hits Selection for Synthetic Lethal RNAi Screen Data

Description

Select hits from synthetic lethal RNAi screen data. For example, there are two identical celllines except one gene is knocked-down in one cellline. The interest is to find genes that lead to stronger lethal effect when they are knocked-down further by siRNA. Quality control and various visualisation tools are implemented. Four different algorithms could be used to pick up the interesting hits. This package is designed based on 384 wells plates, but may apply to other platforms with proper configuration.

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Version

Version

1.2.2

License

GPL-3

Maintainer

Last Published

February 15th, 2017

Functions in synlet (1.2.2)

rankProdHits

Select hits by the rank product method
plateHeatmap

Heatmap of all plates
rsaHits

Select hits by RSA
madSelect

Select hits basing on median +- k*MAD
exampleDat

Synthetic lethal RNAi screen example data.
tTest

student's t-test basing on B-score
bScore

Calculate B-score
zFactor

Calcualte the Z and Z' factor
siRNAPlot

Plot siRNA data and quality metrics.
scatterPlot

Scatter plot of RNAi screen results