Learn R Programming

BitSeq (version 1.16.0)

Transcript expression inference and differential expression analysis for RNA-seq data

Description

The BitSeq package is targeted for transcript expression analysis and differential expression analysis of RNA-seq data in two stage process. In the first stage it uses Bayesian inference methodology to infer expression of individual transcripts from individual RNA-seq experiments. The second stage of BitSeq embraces the differential expression analysis of transcript expression. Providing expression estimates from replicates of multiple conditions, Log-Normal model of the estimates is used for inferring the condition mean transcript expression and ranking the transcripts based on the likelihood of differential expression.

Copy Link

Version

Version

1.16.0

License

Artistic-2.0 + file LICENSE

Maintainer

Antti Honkela

Last Published

February 15th, 2017

Functions in BitSeq (1.16.0)

estimateVBExpression

Estimate expression of transcripts using VB
BitSeq-package

Bayesian Inference of Transcripts from Sequencing data
estimateExpression

Estimate expression of transcripts
loadSamples

Loading and saving expression samples
getMeanVariance

Calculate mean and variance of expression samples
getExpression

Estimate transcript expression
getGeneExpression

Calculate gene expression or relative within gene expression
estimateHyperPar

Estimate hyperparameters for DE model using expression samples and joint mean expression
getDE

Estimate Probability of Positive Log Ratio
parseAlignment

Compute probabilities of alignments
transcriptInfoFile

Manage file containing information about transcript reference
transcriptInfo

Manage information about transcript reference
estimateDE

Estimate condition mean expression and calculate Probability of Positive Log Ratio(PPLR)