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Pasha (version 0.99.21)

Preprocessing of Aligned Sequences from HTS Analyses

Description

Pipeline and additional tools designed to handle aligned data from chromatin NGS experiments (less adapted for typical RNAseq applications). Specifically, starting from aligned reads after sequencing, the pipeline function aims at removing artefacts from datasets, estimate in-silico the fragments size, and generate WIG files with corrected values. The package also provides several tools for WIG files manipulations, scaling, merging and subtract (e.g. input).

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Version

Install

install.packages('Pasha')

Monthly Downloads

39

Version

0.99.21

License

GPL-3

Maintainer

Last Published

March 18th, 2016

Functions in Pasha (0.99.21)

getArtefactsIndexes

Identify reads implied in piles potentially considered as PCR artefacts
.testFunctionalMultiread

Functions dedicated to the functional validation of multiread feature of Pasha package.
generatePiled

Pileup reads in a numeric vector with several piling and elongation options
Pasha-package

Pasha package (Preprocessing of Aligned Sequences from HTS Analyses)
plotReadsRepartitionAnnotations

Graphical information about reads and annotations repartition
normAndSubtractWIG

Normalization and subtraction of WIG files
AlignedData-class

Class AlignedData
WigRepeatAnalyzer

WIG Repeat Analyzer
estimateElongationSize

Insert size estimation in-silico
binVector

Divide a numeric vector in equal bins of signal
multiread_UniformDispatch

Multiread scoring uniform dispatch
elongationEstimation

Shifting the positive strand vector and compute an overlap score to estimate the optimal elongation size for the fragments
pileupDouble

Piling vector of double
mergeWigs

Merge several different wig files in a unique result file
multiread_RemoveArtifact

Artifact Remover
processPipeline

A pipeline to accurately transform aligned reads to genomic enrichment scores
readWIG and writeWIG

Read and Write genomic WIG (fixed step) files
multiread_CSEMDispatch

Multiread scoring CSEM dispatch
.testFunctional

Functions dedicated to the functional validation of Pasha package.