corncob

Count Regression for Correlated Observations with the Beta-binomial

corncob is an R package for modeling relative abundance and testing hypotheses about the effect of covariates on relative abundance. The corncob methodology was specifically developed for modelling microbial abundances based on high throughput sequencing data, such as 16S or whole-genome sequencing.

Installation

To install the corncob package, use the code below to download the development version from Github.

# install.packages("remotes")
remotes::install_github("statdivlab/corncob")
library(corncob)

Docker

Instead of installing corncob to your local system, you can use corncob via the pre-compiled Docker image: quay.io/fhcrc-microbiome/corncob.

Use

The vignettes demonstrate example usage of all main functions (they go over the same analysis, one with phyloseq objects and one without phyloseq objects). Please file an issue if you have a request for a tutorial that is not currently included. You can see the vignette by using the following code:

library(corncob)
# Use this to view the vignette in the corncob HTML help
help(package = "corncob", help_type = "html")
# Use this to view the vignette as an isolated HTML file
utils::browseVignettes(package = "corncob")

Note that R does not allow variable names to start with numbers. Sometimes, when going directly from QIIME2 to phyloseq objects, taxa names will be a large string starting with numbers. To clean these taxa names for use with corncob, use clean_taxa_names(my_phyloseq_object), see ?clean_taxa_names for details.

Documentation

We additionally have a pkgdown website that contains pre-built versions of our function documentation, vignette, and a version of the vignette without phyloseq.

Citation

If you use corncob for your analysis, please cite our manuscript:

Bryan D. Martin, Daniela Witten, and Amy D. Willis. (2020). Modeling microbial abundances and dysbiosis with beta-binomial regression. Annals of Applied Statistics, Volume 14, Number 1, pages 94-115.

An open-access preprint is available on arXiv here.

Bug Reports / Change Requests

If you encounter a bug or would like make a change request, please file it as an issue here.